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10.6 Coupons The group agreed that coupons would be an attractive part of the pilot project, though it was pointed out that coupons would affect project costs and may be seen as a hassle by participating retailers. It was mentioned that coupons might also be used not only to recycle lamps, but also promote the purchase of a new CFL. There have been no offers yet to cover the cost of the coupons. 10.7 Funding The group agreed that the project would benefit by one organization taking a leadership role. It was expressed that the Northwest Energy Efficiency Alliance NEEA ; might be most appropriate due to their receiving funding from utilities that are interested in testing a pilot project and potentially establishing a permanent regional program. Also, in the meeting it was recognized that currently, NEEA is in contact with some 90 utilities and 1, 700 retailers in support of regional programs that make energy efficient products, such as CFLs, available in the marketplace. While a few in the group expressed that CFL recycling may be outside of NEEA's mission, many felt that CFL recycling is in line with NEEA's work in transforming markets because recycling demonstrates responsible end-of-life management of the products they are promoting; and thus represents an appropriate example of `closing the loop'. However, NEEA, although a member of the group, was not represented at the meeting and it is not known whether NEEA would adopt such a program. Further discussion with NEEA was needed to determine if and how the CFL recycling project can contribute to NEEA's mission and work. Some also felt that the pilot model as discussed would underserve rural areas, which are also served by NEEA. The group also explored other sources of funding for the pilot, including working with government and through grant opportunities. The group expressed a desire for all interested parties to work together and contribute in exploring funding options. 10.8 Transition Period During the meeting, the group agreed that a transition period is needed to maintain the momentum of Phase II until development of the pilot project is completed and Phase III is scheduled to begin. The ZWA, having facilitated the project thus far, was nominated to perform the work during this transition period. ZWA expressed willingness in continuing through the transition period.
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Ellmark Blue Cross and Blue Shield of South Dakota members were treated for pneumonia at a higher rate than the national average in 2003. Pneumonia rates for South Dakota members jumped 33 percent between 2002 and 2003. Part of the reason for the high increase could be the severity of the 2003 influenza season. Pneumonia is a complication of influenza. Bronchitis rates in South Dakota, meanwhile, are lower than the national average and only showed a three percent increase between 2002 and 2003. These are some of the findings in the most recent Wellmark Report. In 2005, The Wellmark Report is going to focus on medical trends to help shed light on what is driving costs and utilization for Wellmark Blue Cross and Blue Shield members living in Iowa and South Dakota. The South Dakota communities of Martin, Scotland, Huron, Viborg, Canton, and Flandreau had the highest acute bronchitis episodes, ranging from 147 per 1, 000 in Martin to 97 per 1, 000 in Canton and Flandreau. These rates are well above the national average of 73 per 1, 000. Philip, meanwhile, had the lowest rate of acute bronchitis at 26 per 1, 000, follwed by Hot Springs, Freeman, Pierre, Winner, and Custer. South Dakota communities with the highest pneumonia rates were Huron at 59 per 1, 000, followed by Viborg, Wagner, Dell Rapids, and Mitchell at 47 per 1, 000. Bowdle, meanwhile, had the lowest rate of pneumonia at 13 per 1, 000, followed by Philip, Freeman, Webster, and Gregory. The average cost for treating a case of acute bronchitis was $145.48. Pneumonia is much costlier to treat, with the average cost in 2003 at $517.57. Inpatient expenses are a larger component of pneumonia treatment costs while outpatient and pharmacy expenses drive acute bronchitis treatment costs and raloxifene.
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AbsTr ACT The number of patients taking hMg-CoA-reductase inhibitors for hypercholesterolaemia is growing rapidly. Treatment with hMg-CoA-reductase inhibitors significantly reduces the risk of cardiovascular morbidity and mortality, but may rarely cause serious adverse drug reactions Adrs ; . The most serious Adrs of hMg-CoA-reductase inhibitors are musculoskeletal symptoms including myopathy and myositis, life-threatening ; rhabdomyolysis and liver failure. furthermore, peripheral neuropathy might also occur, especially after long-term use of hMg-CoA-reductase inhibitors. because of the severity and the relative rarity of hMg-CoA-reductase-induced neuropathy, the Netherlands pharmacovigilance Centre lareb has analysed its database of reported Adrs for reports concerning neuropathy associated with the use of hMg-CoA-reductase inhibitors. until June 2005, lareb received 17 reports of neuropathy, peripheral neuropathy and polyneuropathy and in addition two reports of aggravation of existing polyneuropathy associated with the use of hMg-CoA-reductase inhibitors. The associations neuropathy, peripheral neuropathy and polyneuropathy and the use of hMg-CoA-reductase inhibitors are statistically significantly more often reported to lareb. The average time to onset supports conclusions of previous studies and case reports that especially long-term exposure increases the risk for peripheral neuropathy. Considering the increasing number of patients taking hMg-CoA-reductase inhibitors, health care professionals should be aware of the possible role of these drugs in neuropathy and myambutol.
Clinical trials with gugulipid. A new hypolipidaemic agent Nityanand S; Srivastava JS; Asthana OP J Assoc Physicians India India ; May 1989, 37 5 ; p323-8 Multicentric clinical trials of the efficacy of gugulipid conducted at Bombay, Bangalore, Delhi, Jaipur, Lucknow, Nagpur and Varanasi have been reported. Two hundred and five patients completed 12 week open trial with gugulipid in a dose of 500 mg tds after 8 week diet and placebo therapy. One patient showed gastrointestinal symptoms which did not necessitate withdrawal of the drug. A significant lowering of serum cholesterol av. 23.6% ; and serum triglycerides av. 22.6% ; was observed in 70-80% patients Double-blind, crossover study was 414.
A training session is scheduled for local health department staff from 9: 00 a.m. through 1: 00 p.m. on May 6, 2004 via the Public Health Training and Information Network PHTIN ; . This session, entitled Health Check - 2004 Update, will cover the changes in clinical requirements and billing for the Health Check Program. Registration information has been sent to local health departments. If you do not receive this registration information by April 1, 2004, please contact the Public Health Nursing & Professional Development Unit in the Division of Public Health at 919-733-6850. The target audience for this session is both clinical staff who perform the Health Check screenings since the developmental screening changes will have a major impact on the clinical delivery of the Health Check service ; and billing staff. The April 2004 Special Bulletin I, Health Check Billing Guide 2004, is the primary handout for this session. Attendees must access and print the PDF version of this special bulletin from the Division of Medical Assistance's website at : dhhs ate.nc dma bulletin . Copies will not be provided onsite. Joy Reed, Local Technical Assistance and Training Division of Public Health, 919-715-4385 and etoposide and microzide, for example, microzide.
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UVA and UVB decrease the expression of CD44 and hyaluronate in mouse epidermis which is counteracted by topical retinoids E Calikoglu, 2 O Sorg, 1 P Carraux, 1 JH Saurat1 and G Kaya1 1 Dermatology, University Hospital of Geneva, Geneva, Switzerland and 2 Dermatology, Fatih University Medical School, Ankara CD44 is a transmembrane glycoprotein and the principal cell surface receptor of hyaluronate HA ; . In recent study we have shown that two major functions of CD44 in the mouse skin are the regulation of keratinocyte proliferation in response to extracellular stimuli and the maintenance of local HA homeostasis. Recently we have shown that the topical application of retinaldehyde RAL ; resulted in an epidermal hyperplasia and a marked increase of CD44 expression in the epidermis of C57BL 6 and SKH1 hairless mice. Our previous studies have also shown that topical RAL prevented the generation of oxidative stress induced by UVA and UVB irradiation in SKH1 mice. In this study we examined, by anti-CD44 antibody and hyaluronate binding protein HABP ; stainings, the expression of CD44 and HA in mouse epidermis pretreated topically by 0.05% RAL, 0.05% retinoic acid RA ; and 0.05% retinol ROL ; for 3 days and irradiated with UVB, and pretreated by RAL and irradiated with UVA. We also explored the RNA expression of CD44 by Northern blot. Immunostaining of vehicle-treated back skin of SKH1 mice revealed the standard membrane localization of CD44 and HA in basal and suprabasal keratinocytes. CD44 expression was significantly reduced in the membrane and became cytoplasmic 2h after UVA 10 j cm2 ; or UVB 1 j cm2 ; irradiation and reconstituted within 8 or 24 for UVA and UVB, respectively. HA staining and CD44 RNA expression showed a similar pattern of decrease and reconstitution. Topical application of RAL significantly increased the epidermal thickness and the membrane staining of CD44 and HA in follicular and interfollicular keratinocytes and prevented the decrease of CD44 and HA in the epidermis induced by UVA and UVB irradiation. RA and ROL, as RAL, increased the expression of epidermal CD44 at the protein level and of epidermal HA. The role of retinoid-induced increase of epidermal CD44 and HA in the protection of oxidative stress induced by UVA and UVB irradiation is yet to be elucidated.
Table 3: A comparison of active site stereochemistry for selected P450-azole complex structures Cytochrome P450 Resolution ; Ligand1 PDB code Fe A233 C ; Fe S T237 OH ; Fe S T237 C ; Fe A233 C ; Approx. offset2 ; Angular dev.3 ; CYP121 1.9 Fluc 2IJ7 5.49 4.71 CYP51 2.2 Fluc 1EA1 6.24 5.26 CYP119 CYP2B4 1.9 4-Phe 0 20 15 CYP2B4 2.3 Bifo 2BDM 10.72 5.55 0 0.
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158 NEW PHOSPHOORGANIC ANALOGUES OF AMINO ACIDS: OCULOTOXICITY AND ANTIVIRAL ACTIVITY SHIPANOVA AI 1 ; , MAITCHUK YF 1 ; , TOIMELA T 2 ; , ZHUKOV YN 3 ; , OSIPOVA TI 3 ; , KHURS EN 3 ; , KHOMUTOV RM 3 ; 1 ; Moscow Helmholtz Research Institute of Eye Diseases 2 ; Medical School University of Tampere, Finland 3 ; Engelhardt Institute of Molecular Biology, RAS Purpose: evaluation antiviral activity and cytotoxicity of new phosphoorganic analogues of amino acids of general formula R R ; C NH2 ; P O ; OHX, where X H, OH, OR, etc. Methods: in vitro oculotoxicity tests perform with primary pig retinal pigment epithelial RPE ; cell culture, RPE human line D407 ; culture. In vitro tests performed with chicken embryo cell culture being infected with Herpes simplex virus. The new Cell Proliferation Reagent WST-1 by Boehringer Mannhein was chosen to use for cytotoxicity studies. Results: results of study of biological activity of new compounds demonstrated at first time antiviral activity and low cytotoxicity; did not any cytotoxic effect at concentrations higher then biological active concentrations, i.e. in dosages exceeding therapeutic concentrations 10-100 times. The mechanism of biological activity of compounds will be considered in term of substrate or inhibitor properties of phosphoanalogues in relation to enzymes of amino acid metabolism. Conclusion: the perspective of application of compound for ophthalmologic practice as new low cytotoxic antiviral compounds also will be discussed. 159 ALTERED EXPRESSION PATTERNS OF VEGF RECEPTORS IN HUMAN DIABETIC RETINA AND IN EXPERIMENTAL VEGF-INDUCED RETINOPATHY IN MONKEY SCHLINGEMANN RO 1 ; , BLAAUWGEERS HG 1 ; , WEICH HA 2 ; , ALITALO K 3 ; , VRENSEN GF 4 ; , WITMER AN 1 ; 1 ; Ocular Angiogenesis Group, Dept. of Ophthalmology, Amsterdam 2 ; NRCB, Braunschweig, Germany 3 ; Haartman Institute, Helsinki, Finland 4 ; NORI, Amsterdam, The Netherlands Purpose. The vascular endothelial growth factor VEGF ; family is involved in vascular leakage and angiogenesis in diabetic retinopathy DR ; , but may also have physiological functions. Methods. We investigated by immunohistochemistry expression patterns of VEGFR-1, -2, and -3 in relation to leaky microvessels as identified by the marker PAL-E, in retina of 27 eyes of diabetic donors, 18 eyes of non-diabetic controls, and 4 monkey eyes injected with PBS or VEGF-A. Results. In control human and monkey retina, all three VEGFRs were expressed in nonvascular areas, but only VEGFR-1 was constitutively expressed in microvessels. In diabetic eyes, increased microvascular VEGFR-2 expression was found in association with PAL-E ex-pression, whereas microvascular VEGFR-3 was present in a subset of PAL-E-positive cases. In VEGF-A-injected monkey eyes, VEGFR-1, -2, and -3 and PAL-E were expressed in retinal microvessels. Conclusions. The VEGFR-1, -2, and -3 expression patterns in control retinas suggest physiological functions of VEGFs that do not involve the vasculature. Initial vascular VEGF signalling may act primarily through VEGFR-1. In diabetic eyes, expression of retinal VEGFR-2 and -3 is increased, mainly in leaky microvessels. VEGF-A induces vascular expression of the VEGF-A receptor VEGFR-2 and the VEGF-C D receptor VEGFR-3. These findings indicate a dual role of VEGFs in the physiology and pathophysiology of the retina 161 NUCLEIC ACID DELIVERY IN OCULAR CELLS BY LIGHT- SENSITIVE HSV PROTEIN COMPLEXES VALAMANESH F 1 ; , NORMAND N 2 ; , LEFEVRE G 2 ; , MASCARELLI F 2 ; , O'HARE P 3 ; , BEHAR-COHEN FF 1, 2 ; 1 ; Laboratoire d'Innovation Therapeutique en Ophtalmologie, Fondation Ophtalmologique A. de Rothschild, Paris France 2 ; INSERM U450, Paris France 3 ; Marie Curie Research Institute, Oxted United Kingdom Purpose: Evaluate the potential of a new light-sensitive protein vector, VP22 complexes, for the delivery of oligonucleotide into ocular cells in vitro. Methods: The herpes simplex virus 159-301 VP22 protein was incubated with a 20MER fluorescein labelled oligonucleotide Fl-ODN ; leading to the formation of particulate complexes 0.3-1m ; . The complexes were incubated with bovine RPE, RMG cells and with human OCM1 cells ocular choroid melanoma ; overnight. Cells were then washed and incubated in PBS for illumination with either a cold halogen light 250W ; or with a 514nm laser for 4 minutes. Fluoresescence microphotographies were performed before and after illumination. Free Fl-ODN were incubated with the cells as controls. Results: RPE, RMG and OCM1 internalized the particulate complexes. After illumination with either white cold light or with laser, the Fl-ODN dissociated from the particles, distributed homogeneously in the cytoplasma, and concentrated into the nuclei. In the absence of VP22, the naked ODN did not penetrate into cells. No toxic effect of the VP22 complexes on cells was observed using MTT assays. Conclusions: VP22 allows an efficient intracellular penetration of ODN as particules. The illumination of cells loaded with VP22 complexes resulted in the redistribution of ODN in the cytoplasma and the cell nuclei. VP22 has a good potential in ocular gene therapy and eulexin.
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